The aimof this study was to detect the amount of aflatoxinM1 (AFM1) in rawmilk samples in Quchan in northeast of Iran. For this purpose, 34 milk samples were collected from Quchan milk collection centers during November 2012 and analyzed forAFM1 by enzyme linked immune-sorbent assay (ELISA) technique. All the analyses were done twice. Results showed presence of AFM1 in 88.23% of the examined milk samples by average concentration of 65.04ppt and contamination level ranging between 5.33 and 248 ppt. The concentration ofAFM1 in all the sampleswas lower than the Food and DrugAdministration (FDA) limits (500ppt), and, in 13 (38.23%) samples,AFM1 concentrationwasmore than themaximum tolerance limit (50ppt) accepted by European Union (EU) and Codex alimentarius commission (CAC), and in 10 (29.41%) samples,AFM1 concentration wasmore than themaximumtolerance limit accepted by Iranian national standard (100ppt).