According to advanced human diseases and its interest in longevity, the sciences of stem cell biology and repair tissues and damaged organs have been used routinely to develop the cell therapies and regenerative medi- cine for more than three decades. Therefore, the aim of stem cells utilization as a source of appropriate differentiated cells is to replace those lost through physical, chemical or ischemic injury, or as a result of degenerative disease. For this purpose, there are two main kinds of stem cells according to their origin containing embryonic and adult stem cells. Essentially, embryonic stem cells are extracted fetal blood, amnion fluid and membrane or umbilical cord blood (UCB), however adult stem cells are commonly isolated from bone marrow, peripheral blood or skin[6,10]. UCB is a source of hematopoi- etic progenitors cells coupled to the immaturity of the immune system at birth that is one of the advantages of using these cells for transplantation. So the separation of these useful cells requires a standard manual tech- nique or some important protocols and technological innovations. Nowa- days, an accurate and efficient system is the automated closed system “Sepax®” that it is employed largely in the routine processing of cordblood banks worldwide.