All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Comparison of ELISA and HPLC techniques in determination of AFB1 in feedstuff

Author(s): Mohamad Sadegh, Parya Rahnama, Ali Mohamadi Sani

This study aimed to determine the aflatoxin B1 (AFB1) levels in feed-stuff samples. For this purpose, 90 animal feed samples including barley, wheat bran,wheat pulp, canolameal, safflowermeal, cottonseedmeal and sunflower meal were randomly obtained from retail stores in Mashhad-Iran and analyzed by both enzyme linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) techniques. Results showed presence of AFB1 in 33 (36.67%) and 10 (11.11%) samples analyzed by HPLC and ELISAtechniques, respectively. The average±SD concentration ofAFB1 in the sampleswas 2.21±2.25 and 10.76±0.86 µg/kg for HPLC and ELISAtechniques, respectively. The range of contamination reported 0.34- 5.81 and 9.5-12.6 µg/kg for the mentioned techniques respectively. ELISA showed reliability and a high correlation with HPLC of 0.93 indicating its potential for aflatoxin screening in animal feed samples. However, sensitivity and specificity of HPLC was higher than ELISA method.


Share this       
Awards Nomination

Table of Contents

Google Scholar citation report
Citations : 875

BioTechnology: An Indian Journal received 875 citations as per Google Scholar report

Indexed In

  • CASS
  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Cosmos IF
  • Directory of Research Journal Indexing (DRJI)
  • Secret Search Engine Labs
  • Euro Pub
  • ICMJE

View More

Flyer