Malaria is a prevalent disease in India. The results show that the LD50 of the ethanolic extract of Phyllantus amarus and Enicostemma axillare was 500 to 1500 mg/Kg. The in vivo antimalarial activity of the extract against Plasmodium berghei was assessed within 4 days of suppressive test of P. amarus and Enicostemma axillare. The present study involves comparative screening of leaves of phyllanthus and root of Enicostemma for anti-malarial activity on plasmodium berghei induced Swiss albino mice using chloroquine as standard. Ethanolic and aqueus extracts (50, 100 and 200 mg/Kg b.w.) of leaves of phyllanthus and root of Enicostemma axillare were screened administered, produced significant (p < 0.05), dose-dependent activity against the parasite in the suppressive, the present study therefore validates the local use of the extracts of Phyllantus amarus Schumach and Thonn as an antimalarial agent. Further studies are however recommended to identify and possibly characterize the potential antiplasmodial agents in the aqueous extract of the plant.