Antibiotic resistance is common among bacterial pathogens associated with both community acquired and nosocomial infections. In view of the present problem of drug resistance, we investigated the prevalence of methicillin resistant Staphylococcus aureus (MRSA) and amplified the mecA gene in the isolates from the hospital samples. The S. aureus isolates were analyzed for their susceptibility to different classes of antibiotics using Gentamycin and it shows resistance to Kanamycin, Methicillin, Amphotericin and Tetracyclin. MIC test was conducted for S. aureus for antibiotics Ampicillin and Gentamycin. Different samples show different MIC of antibiotics. Plasmid DNA was isolated from the sample 1 and mecA gene was amplified by suitable primers by PCR. This amplified product was sequenced and sequenced size was determined to be 1320 bp. Further it was cloned to plasmid pUC18 vector and transformed to DH5α strains of E. coli. This study highlights the emerging trend of multiple drug resistance in S. aureus samples isolated from hospitals.