Polyhydroxyalkonates a family of polyesters is accumulated as granules in the cells of Pseudomonas putida was significantly depended on the ratio of carbon and nitrogen in the culture medium. The presence of PHA in Pseudomonas putida was confirmed by sudan Black and Nile Blue staining. Kitchen waste and oil waste were used as the renewable raw material for the production of PHA. Highest production of 7.27% was seen in oil waste and 6.3% in vegetable waste. The purified PHA was further confirmed by IR and NMR which showed the presence of the functional groups CO and OH which correlated with the control PHA.