Biofilm is a peculiar characteristic of microorganisms, in which microbial cells stick to each other on a living or non-living surfaces inside a self-delivered grid of extracellular polymeric substance. Extracellular polymeric matrix plays a vital role in structure and function of variety of microbial biofilms. Initial stage of attachment to substratum provides a confined structure, resistivity, metabolic co-operability and genetic traits in microbial community. Biofilms gets affected by some physical factors such as surface of adherence substance, flow of water, quorum sensing etc. This unique feature of microbes help them to habituate in any drastic environmental condition as well as it stimulate their viability.