Bioremediation for elimination of heavy metals gaining much importance through microorganisms. This study was aimed to in-vitro elimination of lead and zinc by using of filamentous fungiAspergillus oryzae.Biosorption of Lead and Zinc by Aspergiilus oryzae was tested at 5 different initial concentrations ranging from 20ppm to 100ppm . The samples were analyzed for decrease in concentrations after 5 days incubation usingAtomic Absorption Spectrophotometer (Chemito AA-203). Aspergillus oryzae showed the maximumpercentage removal of Pb and Zn at 20ppmconcentration and minimum adsorption at 100ppm. It was also observed that removal efficiency of Aspergillus oryzae decreases with increasing concentrations.