Studies were carried out on isolation and identification of bacteria and fungi fromthe clinical specimen and assay the antimicrobial activity (MIC) of essential oil against the clinical isolates. In this study isolated bacterial colonies such as Bacillus subtilis, Escherichia coli, Klebsiella pneumonia, Salmonella typhi, Staphylococcus aureus, Streptococcus pyogenes and Pseudomonas aeruginosa. The KOH and lacto phenol mounting results the isolated fungal colonies were confirmed as Aspergillus flavus, Aspergillus niger, Candida albicans and Trichoderma viridae. The present investigation antibacterial activity (MIC) of essential oil was analyzed against isolated bacteria. The maximum activity was observed in coconut oil and then compared with other essential oil such as peanut oil and sesame oil. The results were compared with standard antibiotics.