Depressive disorder is a prevalent psychiatric disorder, which affects 21% of the world population. The presently using drugs can impose a variety of side effects including cardiac toxicity, hypopiesia, sexual dysfunction, body weight gain and sleep disorder. During the last decade, there is a growing interest in the therapeutic effects of natural products on mental disorders. Antidepressant activity of fractions of chloroform extract of Indigofera barberi was investigated by using Forced swimming test (FST) and Tail suspension test (TST) models. Imipramine was used as reference standard. It has been observed from our study that fraction D of chloroform extract of Indigofera barberi showed significant reduction in immobility in Forced swimming test (FST) and Tail suspension test (TST) models of depression comparable to Imipramine. Further, it is concluded that fraction D is responsible for antidepressant activity.