Plant contains a large numbers of naturally occurring chemicals that have biological activity. Petroleumether extract,Acetone extract, and Methanol extracts of whole plant of Indigofera trita Linn. were prepared and antibacterial activity were studied by disc diffusion method against certain bacterial pathogens such as Escherichia coli, Staphylococcus aureus, Enterobacter aerogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Salmonella typhi, Staphylococcus epidermidis and Proteus vulgaris. The Methanol extracts had wide range of antibacterial activity on these bacterial pathogens than the Acetone extract. Petroleum ether extract were slightly higher antibacterial activity against bacterial pathogens.