All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Anovel approach for the spectrophotometric determination of tryptophan in drug samples of different origins using homemade FIA/ merging zones techniques

Author(s): BushraB.Qassim, Sarah F.Hameed

A new, rapid and sensitive batch and flow injection – merging zones spectrophotometric methods for the determination of Tryptophan in pure material and pharmaceutical preparations were proposed. These methods based on oxidation of the reagent Diphenyl amine sulphonate (DASA) to diphenyl benzidine sulphonic acid after reaction with sodium nitrite in the sulphuric acid medium. The unstable oxidation product react quickly with sodiumnitrite to produce a diazotized intermediate. The diazotized intermediate is coupled with Trp., a pink color product was developed which is stable for 1 h in 300C. The colored product measured at 522nm. Optimum concentration of chemical reactants and physical instrumental conditions have been investigated. A linear graph of peak high versus concentration indicate that BeerÂ’s low is obeyed over the concentration range of 3-40 and 5.100µg. ml-1 of Tryptophanwith detection limits of 0.2325 and 0.188 µg.ml- 1 of Tryptophan for batch and FIA methods, respectively. The optimized FIA system was able to determine Trp. with a through put 52 samples.h-1. The proposed methods were applied successfully for the determination of Tryptophan in pharmaceutical preparations and statistical analysis of the results were compared with results by the British pharmacopeia were also reported.


Share this       
Awards Nomination 20+ Million Readerbase

Table of Contents

Google Scholar citation report
Citations : 378

Analytical Chemistry: An Indian Journal received 378 citations as per Google Scholar report

Indexed In

  • CASS
  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Cosmos IF
  • Electronic Journals Library
  • Directory of Research Journal Indexing (DRJI)
  • Secret Search Engine Labs
  • ICMJE

View More

Flyer