Development of chemical and physical sensors is the current need of society, because from that the recent useful and multipurpose biosensor is made. After the discovery of first biosensor i.e. glucose enzyme electrode in 1962 by Clark and Lyons, there are rapid and many inventions related to the biosensors are done. In the last two decades, we have seen the tremendous development in the field of biosensors, its types and applications in the various areas such as medical diagnostics, food processing, military, environmental monitoring, and biotechnology and among others. In this article detailed information about biosensor and its journey from sense, sensor to biosensor is discussed.