Minangkabau Malays or Minang Malays (Melayu Minangkabau) is one of the Malay subethnic groups in Peninsular Malaysia. Migration of the Minangs from West Sumatra, Indonesia to the state of Negeri Sembilan Darul Khusus in Peninsular Malaysia took place during the late 17th and early 18th centuries, and their descendants now form the main sub ethnic group in this state. For the first time, an analysis of two Alu insertion loci (ACE and TPA25) has been carried out in Minang Malays subethnic group in Peninsular Malaysia. The epithelial buccal cells were collected fromfifty unrelated healthy individuals by using indicating FTAcard and were typed for ACE and TPA25 Alu locus. The PCR amplicons were separated by using 2% (w/v) agarose gel and visualized under UV light. In this study Minang Malays shows trend toward lower ACE and TPA25 Alu insertion, 0.320 and 0.330 respectively. F-stastistic value of 0.2935 suggested that vast genetic differentiation of two different loci existed within Minang Malays in Peninsular Malaysia.