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Ahmed Baker Ali*, Talib Hussen Ali
Biology Department, Education college, Mosul University, (IRAQ) |
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Treatment of male mice with
Nigella sativa alcoholic oily seed extract (0.1, 0.2 and 0.5ml/100g of
Body weight) alone and with combination with UV-radiation ( 366-254nm
wave length) was studied to evaluate its effect on sperm production and
testes tissues morphology. Mice groups were treated with different dose
of Nigella sativa oily extract (0.1, 0.2 and 0.5ml) alone showed an
increase in sperm production and became significantly higher at 0.5ml
concentration after 15 days treatment. Moreover, it showed a
significantly lower(P<0.05) sperm abnormality and sperm death
percentage. The protective effect of different Nigella sativa alcoholic
oily seeds extract concentrations on mice testes irradiated by different
ultraviolet wave length, were investigated. Recovery was not significant
on withdrawal of radiation. However antidotes brought about significant
recovery in the organs studied. Sperm count, sperm abnormality and death
percentage and testes tissue before and after treatment with Nigella
sativa for 2,5,10 and 15days, were recorded. The administration of
different concentrations(0.1, 0 .2 and 0.5ml/100mg of body weight of
alcoholic oily extract of Nigella sativa seeds were significantly able
to minimize the testes cells from being subjected to the degenerative
changes with a concomitant increase in sperm count and significant
decrease in the percentage of abnormal sperms population, depending on
the concentration of treatments, duration and UV- wave length. All three
treatment concentrations brought about significant recovery in the organ
studied. Individually the(0.5ml/ 100mg/body weight) concentration was
the most beneficial in bringing about pronounced recovery .The damage
indicated by UV radiation is transient and reversible. |
