Effect Of GH-RH And Its Analogs On The Secretion Of Interleukin-18 And Tumor Necrosis Factor-a From Human Peripheral Blood Mononuclear Cells In Vitro
 

Agnieszka Siejka¹, Hanna Ławnicka², Tomasz Stępień³,
Roman Krupiński³, Jan Komorowski¹, Henryk Stępień^2*
1Department of Clinical Endocrinology, Chair of Endocrinology,
Medical University of Łódź, 91-425 Lódź, Dr. Sterling 3 St, (POLAND)
²Department of Immunoendocrinology, Chair of Endocrinology, Medical University of Lódź, 91-425 Łódź, Dr. Sterling 3 St, (POLAND)
 ³Department of General and Endocrinological Surgery, Chair of Endocrinology, Medical University of Łódź, 91-425 Lódź, Dr. Sterling 3 St, (POLAND)

The influence of GH-RH and GH-RH synthetic analogs (MZ-4-71, JV-1-65 and JV-1-36) on TNF-α and IL-18 secretion from human peripheral blood mononuclear cells have been studied. GH-RH(1-29)NH₂ and its analogs (JV-1-65 and JV-1-36) stimulated, but GH-RH(1-44)NH₂ and MZ-4-71 inhibited TNF-α secretion. Both GH-RH(1-29)NH₂ and JV-1-65 significantly stimulated the secretion of IL-18. The results demonstrate for the first time the involvement of GH-RH and its analogs in the regulation of IL-18 and TNF-α secretion from human leukocytes.