Antifungal Potency Of Cow Urine
 

H.S.Ravikumar Patil^1*, H.K.Makari², H.Gurumurthy¹, H.L.Ragavendra⁴
D.M.Chetan³, H.S.Anil Kumar^3
1,2Department of Biotechnology, GM Institute of Technology, Davanagere, Karnataka, (INDIA)
²Department of Biochemistry, PG Center Kuvempu University, Tolahunese,
Davanagere, Karnataka, (INDIA)
³Department of Biotechnology, NMAM Institute of Technology, Nitte. Karnataka, (INDIA)

The milk, curd, ghee, urine, and dung of cow are considered to be scared, as they possess some medicinal properties .usage of cow urine in fields confers resistance to plants against pathogens and pests. To give a scientific basis, we collected cow urine from Indian breed cow(malnad gidda) in early morning. The urine was tested against pathogens Fusarium oxysporum, Claviceps purpurea, Rhizopus oligosporus, Asprigulus oryzae obtained from NCIM, Pune, Curvularia spp, Alternaria helianthi and Cladosporium spp were collected from the infected plants parts and the activity was tested against Penillium notatum and Trichoderma viridae. Poison food technique to check the antifungal effect of cow urine. Considerable reduction in colony diameter and extent of sporulation was recorded on Sabouraud’s agar plates. Number of spores germinated in the presence of cow urine was also less when compared to control.. The experiment has given another observation that the older cow urine has got more efficiency than the freshly collected urine samples as it induced stress to the test organism to produce secondary metabolites, which were antimicrobial.