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Nagaraja O.Srikantappa, A.G.Somashekar, G.Malammanavar, M.Krishnappa*
Department of P.G Studies in Applied Botany, Kuvempu University,
Jnanasahyadri Shankaraghatta-577451, Shivamogga, Karnataka, (INDIA) |
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An investigate to detect the
seed-borne fungi of sesame and their effect on germination. Thirty four
fungal pathogens against sesame were conducted in the department of
Applied Botany, Plant pathology laboratory, Kuvempu University
Shivamogga during 2008. A total of twenty eight seed samples were
collected from five taluks of Davanagere district. The seed samples were
also obtained from fields, farmers, retail shops and APMC markets.
Blotter method, potato dextrose agar method, water agar and 2,4-D
methods were used for detection of seed-borne fungi of sesame seeds.
Altogether thirty four fungi belonging to three genera namely
Alternaria alternata, A. sesamicola, Fusarium moniliforme, F. oxysporum,
A. tenuis, Verticillium dahliae, Sclerotinia sclerotiorum, S. rolfsi,
Cercospora sesami, Curvularia lunata, Macrophomina phaseolina,
Cladosporium cladosporioides, C. herbarum, C. fulvum, C. chlorocephalum,
Acremonium sp., Helminthosporium sp., Gliocladium roseum, Neurospora
glabra, Cunigamella elegans, Chaetomium globosum, Stachybotrys chartarum,
S. atra, Pestalotia macrotricha, Aspergillus niger, A. flavus, A.
ochraceus, A. versicolor, A. terreus, A. candidus, Haplosporangium sp.,
Penicillium citratum, Rhizopus nigricans, R, stolonifer and
Mycella sterilliae were isolated from local variety of sesame seeds.
Among all the seed health test methods standard blotter method is most
superior for detection of seed-borne fungi over the other methods. All
the collected seed samples fields and farmers samples shows higher
incidence of pathogenic fungi than others. The effect of seed-borne
fungi on germination capacity was evaluated by sand method and rolled
paper towel method. The associated fungi decreased the germination
potential. All the pathogenic and storage fungi were isolated from
abnormal seedlings, un germinated seedlings and rotted seeds. |