Efficacy Of Bark And Leaf Extracts Of Zizyphus Rugosa Lam. Against
Clinical Bacterial Isolates
 

K.S. Vinayaka^1*, S. Shravanakumar¹, A.C. Roopashree¹, Sandeep M. Otari¹,
S.V. Praveen Kumar², D. Swathi³, T.R. Prashith Kekuda^3
1Dept. of Studies and Research in Applied Botany, Jnanasahyadri, Kuvempu University Shankaraghatta-577451, Karnataka
²Dept. of Studies and Research in Microbiology, Shivagangothri, Tholahunse,
Davangere, Karnataka
³Dept. of Microbiology, S.R.N.M.N College of Applied Sciences, NES Campus,
Balraj Urs Road, Shivamogga-577201, Karnataka

In the present study, the phytoconstituents and antibacterial activity of methanol, chloroform and hexane extracts of bark and leaf of Zizyphus rugosa Lam. was investigated against two pathogens, namely Staphylococcus aureus and Escherichia coli, isolated from clinical specimens were studied. Preliminary phytochemical tests showed the presence of tannins, alkaloids, steroids, saponins and flavonoids in methanol extract of leaves while terpenoids, alkaloids, steroids and saponins were detected in bark extract. All the solvent extracts exhibited antibacterial activity as revealed by zone of inhibition formed around the well. Among isolates subjected, marked inhibition of S. aureus was observed at lesser concentration than E. coli. The leaf extracts were found to inhibit test bacteria at low concentration than bark extracts. Overall, among extracts tested, methanol extract was found to be more potent than other extracts. Methanol and chloroform extracts of leaf and bark inhibited S. aureus at a concentration of 2.5mg/ml while hexane extract revealed MIC of 5mg/ml concentration. The antimicrobial activity of solvent extracts of leaf and bark of Z. rugosa might be possibly due to the presence of the phytoconstituents in the extracts. Since the study involved the clinical isolates, which are among most noted antibiotic resistant bacteria, the extracts could be useful in the treatment of the infections caused by these bacteria. Further experiments are to be conducted to isolate active principles from the extracts and to determine in vivo antibacterial efficacy of phytoconstituents against antibiotic resistant clinical pathogens.